Catalogue Item Name: Ad7h-1
Classification: Adenovirus serotype 7 Genotype: 7h
Passage: 3 Culture Host: A549 cells Time Period Host Cells Infected: 3-5 days
Original Source Information: This isolate was collected in December 1999 from the nasal wash of a 1-year old male in an Iowa hospital.
Method of Identification Confirmation: A virology laboratory performed cell culture and monoclonal fluorescent antibody staining confirmation. The Emerging Pathogens Laboratory repeated cell culture using A549 cells, and also performed microneutralization serotyping using antisera 1-5, 7, and 21. The isolate was determined to be serotype 7. Restriction enzyme analysis of the purified DNA with BamHi revealed a pattern consistent with genotype 7h. (Patterns were compared to five prototype adenovirus serotype 7 of genotypes: 7b, 7d2, 7h, 7p, and 7a, obtained from the CDC).
Reference: M. Noda, T. Yoshida, T. Sakaguchi, Y. Ikeda, K. Yamaoka, and T. Ogino. 2002. Molecular and Epidemiological Analyses of Human Adenovirus Type 7 Strains Isolated from the 1995 Nationwide Outbreak in Japan. J. Clin. Microbiol. 40:140-145.
Biosafety Level: 2
Permits/Forms: A University of Florida Material Transfer Agreement form must be accepted prior to distributing this viral isolate. The point of contact is:
Gary L Heil PhD